| MicroArray Protocol Modified by Ryoji Kojima, Takumi Yoshida and Jeffrey Randall, 8.20.2000 |
| Here are the Basic Steps of the MicroArray Protocol Created by Ryoji Kojima, Takumi Yoshida and Jeffrey Randall on 8.20.2000. |
| 1. Total RNA Isolation from cultured cells. |
| 2. DNase Treatment of Total RNA |
| 3. Making the single strand cDNA probe. |
| 4. Automated Slide Processor (ASP) Version for hybridization. |
| 5. Washing microarrays in ASP. |
| 6. Processing of Array slide |
| 7. Pre-hybridization of the processed slides (NON-Automated version). |
| 8. Hybridization of Cy3 + Cy5 probe to glass array (NON-Automated version). |
| 9. Preparation of Dendrimer Cy3 and Cy5. |
| 10. Washing unbound probe from glass array (NON-Automated version). |
| 11. Hybridization of Dendrimers (Cy3 and Cy5) to Array (NON-Automated version). |
| 12. Washing unbound dendrimer from glass array(NON-Automated version). |
| To View and Print an Adobe® PDF of the MicroArray Protocol click Here. |
